Epigenetic & reparation
Publié le 18/11/2022
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Chromatin and DNA repair in yeast
Bouvier for G.
Almouzni Chromatin and DNA repair in yeast - INTRODUCTION: DNA damaging agents/DNA repair pathways DNA lesions in the context of chromatin A yeast model to study response to DSB Double Strand Break Detection and Signaling in S.
Cerevisiae Double Strand Break repair in S.
Cerevisiae - The histone code for DNA signaling and repair - ATP-dependent nucleosome remodeling factors in DSB repair - DNA lesions in the context of the Nucleus - DSB intranuclear localisation in S.
cerevisiae Art: N.
Bouvier for G.
Almouzni DNA damaging agents and DNA repair pathways from J.
Nature (2001). DNA lesions in the context of chromatin 1.
First evidence from UV irradiation -photoproducts, persist in tracts of DNA which are rendered refractory to excision repair by a 'mask' of protein (Wilkins and Hart, 1974) The effect of chromatin structure on repair was first implicated by the observations of Wilkins and Hart (R.J.
Hart, 1974) that UV induced lesions in permeable human cell chromatin are less accessible to CPD cleavage enzymes in M.
luteus extracts than permeable cells treated with high salt (to remove histones). -UV induces chromatin relaxation (Smerdon et al., 1978) Rubbi and Milner, 2003 Rubbi and Milner, 2003 DNA lesions in the context of chromatin 2.
The Access, Repair, Restore model
(Smerdon, 1991 and Green and Almouzni, 2002 )
(Groth et al., 2007)
A yeast model to study response to DSB
3 MATa Tel Cen Tel HML/R strain " inducible HO HML Chr.
3 Tel MATa Cen HMR Tel Double Strand Break Detection and Signaling in S.
Cerevisiae - G2/M arrest - DNA damage inducible genes - RNR regulation Double Strand Break repair in S.
Cerevisiae Homologous Recombination Non Homologous End Joining Van Attikum and Gasser(2005) The histone code for DNA signaling and repair Van Attikum and Gasser(2005) The histone code for DNA signaling and repair P Mec1ATR/Tel1ATM The last serine of C-term of H2A or variants is well conserved and rapidly phosphorylated around DSBs (Downs et al.,2000) IMR90 normal human fibroblasts Unirradiated (Shroff et al., 2004) 3 min after 0.6Gy (Rocagou et al., 1999) The histone code for DNA signaling and repair P Mec1ATR/Tel1ATM An assembly platform rather than a recruitment signal for repair and signaling proteins: -MRX complex -Rad9 -Cohesins -Histone modifiers (NuA4 histone acetyl transferase) -Chromatin remodelers (INO80, SWR1) 53BP1 foci (Celeste et al., 2003) The histone code for DNA signaling and repair H2O2 induced apoptosis (Ahn et al., 2005) P Ste20MST1 Ub repair (Driscoll et al., 2007) replication (Han et al., 2007) Hat1 HR (Qin.... »
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